mouse leptin receptor lepr biotinylated antibody  (R&D Systems)

Journal: Aesthetic Plastic Surgery

Article Title: A Rat Model Investigation of Enhanced Facial Rejuvenation via PCL Microsphere-Induced Superior Collagen Neogenesis in the Supraperiosteal Plane

doi: 10.1007/s00266-025-05503-6

Figure Lengend Snippet: Effect of implanted fillers on the recruitment of PSCs and ingrowth of blood vessels following implantation in the periosteum of rat skull for a duration of 1 month. Scale bars, 100 μm. A Representative immunostainings of LepR+ PSCs, Emcn+ vessels, and OPN+ mature osteoblast in HA and PCL fillers. B , C Quantification of the relative Emcn+ vessel area in implants ( B ) and peri-implants ( C ). D Quantification of the relative LepR+ area in implants. Data are means ± SD. * P < 0.05, and *** P < 0.005 (unpaired two-tailed Student’s t test)

Article Snippet: Mouse Leptin Receptor (LepR) biotinylated antibody (BAF497) was sourced from R&D systems (Minneapolis, MN, USA).

Techniques: Two Tailed Test

Journal: JBMR plus

Article Title: Enzyme-Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells.

doi: 10.1002/jbm4.10722

Figure Lengend Snippet: Fig. 5. Modified bone marrow transplantation (c-BMT) method isolated the bone marrow stromal cells more than the normal BMT method. Scatter plots from flow cytometry analysis of isolated bone marrow cells depicting CD45/Ter119/CD31-positive and LepR+ (A), CD51+ (B), and RUNX2+ (C). Left panel: BMT; right panel: c-BMT. Numbers indicate the percentage of LepR+, CD51+, and RUNX2+ cells in the total population.

Article Snippet: Cells (1.0 107) were suspended in 100 μL of 2% FBS/PBS and then incubated with anti-leptin receptor (LepR) biotinylated antibody (R&D Systems, Minneapolis, MN, USA; BAF497, 1:100), anti-CD51 antibody (BioLegend, San Diego, CA, USA; 104105, 1:100), anti-CD45 antibody (BioLegend, 103111, 1:100), anti-TER-119 antibody (BioLegend, 116211, 1:100), and anti-CD31 antibody (BioLegend, 102509, 1:100) for 60 minutes on ice.

Techniques: Transplantation Assay, Isolation, Cytometry

Journal: PLoS Genetics

Article Title: Congenital lipodystrophy induces severe osteosclerosis

doi: 10.1371/journal.pgen.1008244

Figure Lengend Snippet: A) Transplanted fat depots 3 months after surgery. B) Fat depot weight 3 months after transplantation. C) Serum leptin and adiponectin of FF mice 3 months after fat depot transplantation. WT and non-transplanted FF mice serve as control. D) μCT analysis of trabecular bone volume and bone mineral density of distal femurs of FF mice 3 months after sham operation or transplantation of fat derived from WT or adipokine-deficient mice. Data are presented as mean ± SD. **p<0.01; *** p<0.001 as determined by ANOVA with Holm-Sidak's post hoc analysis for multiple comparisons test. D) comparison with FF Sham except where detailed.

Article Snippet: The primary antibody cocktail contained rat anti-mouse CD45-BUV395 (BD Horizon, clone 30-F11, final dilution factor 1:200), rat anti-mouse TER-119-APC (BioLegend, clone TER-119, 1:200), rat anti-mouse CD41-BV421 (BioLegend, clone MWReg30, 1:300), rat anti-mouse/human CD11b (BioLegend, clone M1/70, 1:400), and rat-anti mouse Leptin receptor (LepR)-biotin (R&D Systems, polyclonal, 1:50) in Brilliant Stain Buffer (BD Biosciences) containing 10 μg/mL FcBlock.

Techniques: Transplantation Assay, Control, Derivative Assay, Comparison